Which Statement Regarding the Gram Stain Is False

Select ALL applicable explanations for a false negative Gram staining the bacterium is Gram positive but appears negative on the slide Answers. Because they dont hold the dye.

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Brownish waxy colonies grew on the blood agar.

. Escherichia coli stains pink because it has a thin peptidoglycan layer b. Are more abundant in the human body than human cells. Acetone-alcohol decolorizes gram-negative bacteria.

Only include members of the tree of life. A they are found as rods spheres or spirals. Mycobacteria stain weakly with gram stain and bacteria such as Mycoplasma Rickettsiae Chlamydiae do not take up the dyes used in Gram stain or are too small to be seen with light microscopy.

We quantify the false-negative diagnostic rate of septic arthritis using Gram-stain microscopy of synovial fluid and compare this to values reported in the peer-reviewed literature. Visualization of a Gram-negative bacterium or a non-acid-fast bacterium is due to the uptake of the counterstain by the cells. Gram-positive begin to appear as gram negative.

Each of the following statements concerning the Gram stain is correct except. Simple stains reveal information about size shape and arrangement. In most cases Gram staining is not sufficient to identify an organismfor example Gram-positive staphylococci on skin may be either S.

Gram stain is the most important staining procedure. Coli 0157H7 produces clear colonies on MacConkey agar while common strains of the organism produce pink colonies on this medium. Sensitivity of Gram Staining Technique.

Coli from Salmonella enterica. Porins allow entry of substances into both Gram-positive and Gram-negative bacteria. B They are never anaerobic.

Staining the cells of a heat-fixed smear with just methylene blue would be considered a differential stain. Gram-positive bacteria stain purple whereas gram-negative bacteria stain pink. Indicate the true statements and then correct the false statements so that they are true.

Gram-negative bacteria lack any peptidoglycan so they are not stained by the dye. So the correct option is True. C They contain a peptidoglycan cell wall with the exception of some bacteria that lack cell wall.

Bacterial culture with older cells more than 24 hours lose Gram positivity and will appear as Gram-negative. The most likely cause of the infection is a A gram-negative bacterium. We propose a method of improving the diagnostic value of Gram-stain microscopy using Lithium Heparin containers that prevent synovial fluid coagulation.

Limitation of Gram Stain. On the same day a smear was made for Gram staining. Which of the following statements is true.

Safranin stains the gram-negative bacteria red. Slide cultures set up on February 1 and incubated at 25C showed septate hyphae and single conidia. Dark field microscopy requires a stained sample.

Regarding the gram stain which one of the following is the most accurate. The Gram stain is a simple stain. Grams iodine is used to bind the primary stain.

The Gram stain showed large 10 µm cells. Which statement regarding the Gram stain is FALSE. The counterstain for the acid-fast stain is carbol fuchsin.

View the full answer. Gram-positive bacteria have a single cell wall anchored to the cell membrane by lipoteichoic acid. The cell wall of Gram-negative bacteria is thick and the cell wall of Gram-positive bacteria is thin.

Distilled water is used for the washing steps. E They reproduce by binary fission. A Gram stain B acid-fast stain C endospore stain D negative stain 32 Which of the following is a FALSE statement concerning simple stains.

Share a recent common ancestor. D They are found as single cells or arranged in different ways. The negative stain is a type of differential stain.

Crystal violet stains the gram-positive bacteria purple. Question 49 2 points Which of the following statements regarding the Gram staining of bacterial cells is False Gram negative cells have a thin layer of peptidoglycan in their cell wall Gram positive cells have a thick layer of peptidoglycan in their cell wall O Gram negative cell stain purple Gram positive cells stain purple Question 50 2. Which of the following statements regarding bacteria is False.

Streptococcus pyogens stains blue because it has a thick peptidoglycan layer c Mycoplasma pneumoniae is not visible in the Grams stain because it does not have a cell wall d. Bright field microscopy requires a stained sample. Which statement is false when a Gram stain is performed.

To be visible on a slide organisms that stain by the Gram method must be present in concentrations of. The application of too much heat during fixation can lead to loss of gram positivity. A They are composed of a single basic dye B Example of a simple stain - Methylene blue C Simple stains make use of more than one dye to distinguish between cells D They can be used to determine the.

After adding the crystal violet and grams iodine both gram-positive bacteria and gram-negative bacteria will appear blue. The age of bacterial culture. Excessive heat destroys cell wall.

Gram staining can be used to differentiate intestinal normal microbiota from pathogensfor example differentiating E. Medically important bacteria can seldom be identified by Gram staining alonefor example the throat may harbor Streptococcus pyogenes pathogen as well as Streptococcus species that are normal. Cannot survive in boiling water.

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